Aislamiento, identificación molecular y actividad enzimática de bacterias termófilas productoras de amilasa de aguas termales
DOI:
https://doi.org/10.5935/jetia.v7i30.771Resumen
En esta era, la exploración de enzimas en Indonesia continúa aumentando y la enzima amilasa de aproximadamente el 30% de la producción total se utiliza en todo el mundo. Las grandes necesidades de la enzima amilasa y sus usos en diversos campos industriales y sanitarios llevan a la búsqueda de potenciales recursos naturales en su producción, entre ellos las aguas termales, muy prometedoras por sus propiedades termofílicas y termoestables. El objetivo de este estudio fue aislar, determinar la actividad y la identificación molecular de bacterias termófilas productoras de amilasa de las aguas termales naturales Dolok Tinggi Raja, Simalungun, Sumatra Norte. Este estudio incluye la medición de la actividad enzimática mediante el método DNS y la identificación molecular. Los resultados se obtuvieron a partir de 20 aislamientos aislados, 6 aislamientos tuvieron actividad amilasa con la actividad más alta, a saber, el aislado UTMTR VAR A10 con una actividad enzimática amilasa de 0.2733 Unidad / mL. Con base en la identificación molecular usando amplificación del gen 16SrRNA basada en el programa BLAST y análisis filogenético con el programa MEGA X, se demostró que el aislado UTMTR VAR A10 es una bacteria Bacillus licheniformis cepa UTMTR VAR A10. Se necesitan más investigaciones para purificar la enzima de tal manera que pueda aplicarse al mundo industrial.
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